Question: We must not put the protein in the well by itself but rather
first mix it with denaturing buffer….
We must not put the protein in the well by itself but rather
first mix it with denaturing buffer. It is important to denature
the protein with this buffer and by heating before running the
sample on an SDS-PAGE because this way the 3D shape does not
influence the rate at which the protein migrates and the gel
results are due to size only. You are using a 4X sample buffer.
This means that you must dilute it 4-fold to reach your final
sample concentration (V2/V1 = 4). You plan to add 10 μL of sample
buffer to your sample. What volume of protein solution should you
add the sample buffer to in order to have the final concentration
of the sample buffer be 1X