Question: Studied MF in a simple way. E.coli are sensitive to (killed by) the antslsolic rifamycin Grif o r…


studied MF in a simple way. E.coli are sensitive to (killed by) the antslsolic rifamycin Grif o rif) However approximatkly 1V10 -10E col are resdistant to nfamycin Gi)which can be the resalt of a variety of has on mutations in a particular gene (rpe Studying changes in mutation frequency i.e, increases t which the E. coli genome is being mutated. Mutation frequency is simply the a from 109) for rif rif gives a measure of the rale at celi geaome is being mutated. Mutation frequency is simply the ratio lnlVol t L s mutation frequency was studied in a variety of cells with diflerent genotypes (Table D. The mutH gene encodes the MutH prolein (etc) Table I Cenot Spontaneous Mutation Frequency 1x10 1x10-7 1x10 x10 what you know about these genes, explain the results in Table I. Be sure to explain explicily what each of these genes does There is one gene in this table that you have not heard about. Simply mention which it is in will have to conclude something aboot this gene afier you consider the results in Table IL. Several other strains of E.coli-notably double mutants-were also tested (Table I1) Table II Genotype of E.colf Spontaneous Mutation Frequency 1x10 muth /mutl /muts- ImutY/dam mutH-/mutl /muts /mutY-/dam 1x10-7 1x10 2x10-7 111.2.b. Rationalize the results in Table I1. In the next data set (Table I11), a strain of E. coli was used whose genomic DNA was mut N IS rytidamt (wild type) in each case. To this cell was added a multi-copy plasmid that contained a cloned copy of a g mutht gene in the second line. In each case, the cloned gene is expressed normally, although this multi-copy plasmids was present at-40 copies per cell. In cach of the entries in Table III, mutation frequency was measured Spontaneous Mutation Frequency Genotype of E.coli mutH /L /s /Y/dam + no plasmid +muth containing plasmid + mut containing plasmid +muts containing plasmid + mut containing plasmid + dam containing plasmid 1x10-9 1x10-9 0.5x10-9 1x10-9 1x10-9 1x10-7 III.2e. Rationalize the results in Table I11.

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studied MF in a simple way. E.coli are sensitive to (killed by) the antslsolic rifamycin Grif o rif) However approximatkly 1V10 -10E col are resdistant to nfamycin Gi)which can be the resalt of a variety of has on mutations in a particular gene (rpe Studying changes in mutation frequency i.e, increases t which the E. coli genome is being mutated. Mutation frequency is simply the a from 109) for rif rif gives a measure of the rale at celi geaome is being mutated. Mutation frequency is simply the ratio lnl'Vol t L s mutation frequency was studied in a variety of cells with diflerent genotypes (Table D. The mutH gene encodes the MutH prolein (etc) Table I Cenot Spontaneous Mutation Frequency 1×10 1×10-7 1×10 x10 what you know about these genes, explain the results in Table I. Be sure to explain explicily what each of these genes does There is one gene in this table that you have not heard about. Simply mention which it is in will have to conclude something aboot this gene afier you consider the results in Table IL. Several other strains of E.coli-notably double mutants-were also tested (Table I1) Table II Genotype of E.colf Spontaneous Mutation Frequency 1×10 muth" /mutl /muts- ImutY/dam mutH-/mutl /muts /mutY-/dam 1×10-7 1×10 2×10-7 111.2.b. Rationalize the results in Table I1. In the next data set (Table I11), a strain of E. coli was used whose genomic DNA was mut N IS rytidamt (wild type) in each case. To this cell was added a multi-copy plasmid that contained a cloned copy of a g mutht gene in the second line. In each case, the cloned gene is expressed normally, although this "multi-copy" plasmids was present at-40 copies per cell. In cach of the entries in Table III, mutation frequency was measured Spontaneous Mutation Frequency Genotype of E.coli mutH /L /s /Y/dam + no plasmid +muth containing plasmid + mut containing plasmid +muts containing plasmid + mut containing plasmid + dam containing plasmid 1×10-9 1×10-9 0.5×10-9 1×10-9 1×10-9 1×10-7 III.2e. Rationalize the results in Table I11.

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