Question: Question 2 A cross was made between an Hfr donor E. coli strain that is met thit pur and an F rec…


Question 2 A cross was made between an Hfr donor E. coli strain that is met thit pur and an F recipient strain that is mef thí pur. An interrupted mating experiment showed that met enters the recipient last. In a separate experiment the Hfr X F cross was repeated, allowing sufficient time for met to be transferred. Recombinant recipients were selected on medium lacking methionine (selecting for met) but containing both thiamine and purines (not selecting for thi or pur). The met recombinants were ther subsequently tested the presence of the thi and pur alleles. The following table shows the number of recombinant clones with each genotype. met thi pur 280 met thi pur 52 met thi pur met thi pur What is the order of these genes relative to each other and the Hfr origin of transfer? Your map should have the following form in the Blackboard answer text box, where x, y, and z indicate the gene names, and > indicates the Hfr origin and direction of transfer:

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Question 2 A cross was made between an Hfr donor E. coli strain that is met thit pur and an F recipient strain that is mef thí pur. An interrupted mating experiment showed that met enters the recipient last. In a separate experiment the Hfr X F cross was repeated, allowing sufficient time for met to be transferred. Recombinant recipients were selected on medium lacking methionine (selecting for met') but containing both thiamine and purines (not selecting for thi or pur). The met recombinants were ther subsequently tested the presence of the thi and pur alleles. The following table shows the number of recombinant clones with each genotype. met thi pur 280 met thi pur 52 met' thi pur met' thi pur What is the order of these genes relative to each other and the Hfr origin of transfer? Your map should have the following form in the Blackboard answer text box, where x, y, and z indicate the gene names, and > indicates the Hfr origin and direction of transfer:

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