Question: L T-Mobile LTE 5:39 PM Bio 302 Prob Set-Mapping.doc Гђ Bio 302 Prob Set-Mapping.doc Bio 302-Probl…


l T-Mobile LTE 5:39 PM Bio 302 Prob Set-Mapping.doc Гђ Bio 302 Prob Set-Mapping.doc Bio 302-Problem Set -Mapping Prof. Small 1) (14 pts) You have discovered a new species of worms for which the haploid number is three. You isolated a dumpy wornm and want to determine the linkage group of the gene dpy-1. The dpy-1 mutation is recessive. Using recessive marker mutations for each chromosome (i.e, marker 1 is on chromosome I marker 2 is on chromosome II, marker 3 is on chromosome II) you set-up 3 crosses as follows: Cross I: marker mutation 1 x dpy-1 Cross 2: marker mutation 2 x dpy-1 Cross 3: marker mutation 3 x dpy-1 Fl progeny for the different crosses are allowed to mate, and you select the animals carrying the marker mutation from this cross. You find the following results: Of 300 marker mutation 1 animals, 200 are dpy-1 Of 300 marker mutation 2 animals, 150 are dpy-1 Of 300 marker mutation 3 animals, 13 are dpy-1 a) On which chromosome is dpy-1? You now wish to determine the position of dpy-1 on the chromosome. You have identified several other recessive mutations on that chromosome. These other mutations include eyeless and hairy. You have a female worm that is heterozygous for eyeless hairy, and dpy-1 and testeross the animal (i.e., mate the het to an animal homozygous for eyeless, hairy, dpy-1). Among the 1000 Fl progeny from the testcross, the following phenotypes were observed Dpy-1, eyeless, hairy wild type hairy, dpy-1 eyeless eyeless, hairy dpy-1 eyeless, dpy-1 hairy 450 470 30 40 34 36 b) What is the order of genes on the chromosome and the map distances between the genes?

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l T-Mobile LTE 5:39 PM Bio 302 Prob Set-Mapping.doc Гђ Bio 302 Prob Set-Mapping.doc Bio 302-Problem Set -Mapping Prof. Small 1) (14 pts) You have discovered a new species of worms for which the haploid number is three. You isolated a dumpy wornm and want to determine the linkage group of the gene dpy-1. The dpy-1 mutation is recessive. Using recessive marker mutations for each chromosome (i.e, marker 1 is on chromosome I marker 2 is on chromosome II, marker 3 is on chromosome II) you set-up 3 crosses as follows: Cross I: marker mutation 1 x dpy-1 Cross 2: marker mutation 2 x dpy-1 Cross 3: marker mutation 3 x dpy-1 Fl progeny for the different crosses are allowed to mate, and you select the animals carrying the marker mutation from this cross. You find the following results: Of 300 marker mutation 1 animals, 200 are dpy-1 Of 300 marker mutation 2 animals, 150 are dpy-1 Of 300 marker mutation 3 animals, 13 are dpy-1 a) On which chromosome is dpy-1? You now wish to determine the position of dpy-1 on the chromosome. You have identified several other recessive mutations on that chromosome. These other mutations include eyeless and hairy. You have a female worm that is heterozygous for eyeless hairy, and dpy-1 and testeross the animal (i.e., mate the het to an animal homozygous for eyeless, hairy, dpy-1). Among the 1000 Fl progeny from the testcross, the following phenotypes were observed Dpy-1, eyeless, hairy wild type hairy, dpy-1 eyeless eyeless, hairy dpy-1 eyeless, dpy-1 hairy 450 470 30 40 34 36 b) What is the order of genes on the chromosome and the map distances between the genes?

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