Question: It’s a practice question. Can someone help walk me through
this so I know how to do it?!


It’s a practice question. Can someone help walk me through
this so I know how to do it?!
The diagram above represeHl for a redigestion. However, in practice, this approaci tubes are set up-one containing Eco RI, one with Hind III, a Hindl lI, so that a simultaneous double digest nd the l l occurs. The following lists show whl might look like for a DNA approximately the size of Lambda: Hind III 23100 5350 5100 4750 2700 Eco RI Eco Ri and Hind Ill 21200 4400 21200 7450 6200 4300 3700 2700 1900 5800 350 1400 1050 350 The digest a 1,900 Eco RI 21,200 fragment must overlap with the Hind III 23,100 fragment, so that in the double fragment is produced (23,100-21,200 1,900). This result means the next Eco RI fragment also contains that 1,900-nucleotide sequence, so that the 1,900 fragment plus another dou- ble-digest fragment must equal one of the Eco RI fragments. This turns out to be the 4,300 fragment 1,900 plus 4,300 equals the 6,200 Eco RI fragment. The next Hind III fragment also contains the 4,300 is base pairs, so 4,300 plus a double-digest fragment must total to equal a Hind III fragment. Use scrap paper and map this DNA; then, draw your map in the space provided below and indi- cate where each of the restriction sites is.

Show transcribed image text

The diagram above represeHl for a redigestion. However, in practice, this approaci tubes are set up-one containing Eco RI, one with Hind III, a Hindl lI, so that a simultaneous double digest nd the l l occurs. The following lists show whl might look like for a DNA approximately the size of Lambda: Hind III 23100 5350 5100 4750 2700 Eco RI Eco Ri and Hind Ill 21200 4400 21200 7450 6200 4300 3700 2700 1900 5800 350 1400 1050 350 The digest a 1,900 Eco RI 21,200 fragment must overlap with the Hind III 23,100 fragment, so that in the double fragment is produced (23,100-21,200 1,900). This result means the next Eco RI fragment also contains that 1,900-nucleotide sequence, so that the 1,900 fragment plus another dou- ble-digest fragment must equal one of the Eco RI fragments. This turns out to be the 4,300 fragment 1,900 plus 4,300 equals the 6,200 Eco RI fragment. The next Hind III fragment also contains the 4,300 is base pairs, so 4,300 plus a double-digest fragment must total to equal a Hind III fragment. Use scrap paper and map this DNA; then, draw your map in the space provided below and indi- cate where each of the restriction sites is.

(Visited 1 times, 1 visits today)
Translate »