Question: Indicate what the EcoRt sitc looks ike when cach is aive. 2 Fx each of the following blotung tec …


indicate what the EcoRt sitc looks ike when cach is aive. 2 Fx each of the following blotung tec haiques, completc this tabde in your Blue Book If in any case muduple possability Ma esulc in ssl Tyis.of Fks Souilera bloi 3. A 5000bp plassnid with no nicks is compleicly B DNA and has 25 undkerwindings What are L,T, W and AL What are the three anost impotant uses for acgaive superhclical torns in the cell? (Do NOT use Z-DNA as one of the aaswers.) 5. Name the kind of proicin that: a. Separates DNA strands b. Syathesizes RNA primers c Stabilizes ss DNA (in bacteria) 6. a The DaaA protcia exists in an active and inactive form, which differ by binding to what small molecule(s)? b. The active form is? c. DnaA bound at oriC is converted to the inactive form by a process called what? d. DnaA NOT bound at oriC is converted to the inactive form at a site called what? 7. The timing of initiation of lcading strand syathesis in E.coli can vary depending on growth condiions. a. Two small molecules can have an effect on initiation of replication. Name the small molecule that is derived in part from GTP (either of its two names is sufficicnt) b. This small molecule is thought to affect DNA replication by modulating the expression of a genets). Name it (them). c. la one sentence explain how the modulation of the gene (or onc of the genes) you picked in 7.b. affects replication initiation. 8. Draw a figure (low chart) showing the steps during nornal replication termination in E. coli, beginning after the clockwise and counter-clockwise replication forks encounter each other. 9. The E.coli chromosome is compacted by binding to a variety of proteins. a. Name the protein (or one of the protcins in the complex) that binds DNA to give -340bp loops. b. This protcin (or protein complex) is a member of what family of proteins? c. What cukaryolic ortholog is also in this family? 10. This concerns the fate of a lesion formed in DNA (c.g. a thymine dimer). If the DNA damage (lesion) is not repaired rapidly, then it might be encountered by a DNA replication fork, which leads to a structure that cannot boe repaired by a DNA repair pathway (usually) a. What two cellular processes (or pathways or mechanisms) does the cell have to deal with this situation? b. What is the gencral name given to these two processes? IL.a. Name one protcin that repairs 6-methylguanine (and 4-mcthylthymine). b. Beginning with omeG, draw the mechanism. Do not draw guanine; simply begin with (CH-G DNAL a. When a glycosylase acts on DNA damage it leaves b. The protcin that acts on the entity mentioned in 12a. is named c. The proteins in 12a. and 12b are part of the DNA repair pathway named 12. in DNA.

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indicate what the EcoRt sitc looks ike when cach is aive. 2 Fx each of the following blotung tec haiques, completc this tabde in your Blue Book If in any case muduple possability Ma esulc in ssl Tyis.of Fks Souilera bloi 3. A 5000bp plassnid with no nicks is compleicly B DNA and has 25 undkerwindings What are L,T, W and AL What are the three anost impotant uses for acgaive superhclical torns in the cell? (Do NOT use Z-DNA as one of the aaswers.) 5. Name the kind of proicin that: a. Separates DNA strands b. Syathesizes RNA primers c Stabilizes ss DNA (in bacteria) 6. a The DaaA protcia exists in an active and inactive form, which differ by binding to what small molecule(s)? b. The active form is? c. DnaA bound at oriC is converted to the inactive form by a process called what? d. DnaA NOT bound at oriC is converted to the inactive form at a site called what? 7. The timing of initiation of lcading strand syathesis in E.coli can vary depending on growth condiions. a. Two small molecules can have an effect on initiation of replication. Name the small molecule that is derived in part from GTP (either of its two names is sufficicnt) b. This small molecule is thought to affect DNA replication by modulating the expression of a genets). Name it (them). c. la one sentence explain how the modulation of the gene (or onc of the genes) you picked in 7.b. affects replication initiation. 8. Draw a figure (low chart) showing the steps during nornal replication termination in E. coli, beginning after the clockwise and counter-clockwise replication forks encounter each other. 9. The E.coli chromosome is compacted by binding to a variety of proteins. a. Name the protein (or one of the protcins in the complex) that binds DNA to give -340bp loops. b. This protcin (or protein complex) is a member of what family of proteins? c. What cukaryolic ortholog is also in this family? 10. This concerns the fate of a lesion formed in DNA (c.g. a thymine dimer). If the DNA damage (lesion) is not repaired rapidly, then it might be encountered by a DNA replication fork, which leads to a structure that cannot boe repaired by a DNA repair pathway (usually) a. What two cellular processes (or pathways or mechanisms) does the cell have to deal with this situation? b. What is the gencral name given to these two processes? IL.a. Name one protcin that repairs 6-methylguanine (and 4-mcthylthymine). b. Beginning with omeG, draw the mechanism. Do not draw guanine; simply begin with (CH-G DNAL a. When a glycosylase acts on DNA damage it leaves b. The protcin that acts on the entity mentioned in 12a. is named c. The proteins in 12a. and 12b are part of the DNA repair pathway named 12. in DNA.

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