Question: Indicale what the EcokI sate 2. For cach of the following blosung tew.hnius, complcte answers are…


indicale what the EcokI sate 2. For cach of the following blosung tew.hnius, complcte answers are possible, jusl give onac possabulity this taldc in your Bluc Book in any case muliple a Southcrn blot Masromalcul ingsi Tys.oruls 3. A 5000bp plasmid with no nicks is compleily B DNA and has 25 wnkerwindings What are L,T.W 4. What are the threce most impxtant negalive answers) 5. Name the kind of proicin thal: and AL taat uses for egaavc sapcihelsal turus··thc cell? (Do NOT use Z-DNA as one d the a. Separates DNA strands b. Synthesizes RNA primers 6. a. The DaaA protcia cxists in an active and inactive form, which dilfer by binding to what small molecule(sy? b. The active form is? c. DaaA bound at oriC is converted to the inactive form by a process called what? d. DaaA NOT bound at oriC is converted to the inactive form at a site called what? 7. The timing of initiation of leading strand syathesis in E.coli can vary depcading on growh conditions in part from GTP (cither of its two aames is sufficient) (them) replication initiation. 8. Draw a figure (low chart) showing the steps during nonnal replication termination in E.coli, beginning after the a. Two sanall เ¡olecules can have an eflect on inaliauon of replicatia Name the small molecule that is derived b. This small nule cule is เ1ought lo allect DNA replication by anodulating thc cupression of a gcne(s), Name i c. la one sentence explain how the modulation of the gene (or one of the genes) you picked in 7.b. affects clockwise and counter-clockwise replication forks encounter each other. 9. The E.coli chromosome is compacted by binding to a variety of proteins. a. Name the proteia (or one of the proteins in the complex) that binds DNA to give -340bp loops b. This protcia (or prolein complex) is a member of what family of proteins? c. What cukaryotic ortholog is also in this family? 10. This concerns the fate of a lesion formed in DNA (e.g. a thymine dimer). If the DNA damage (lesion) is not repaired rapidly, then it might be encountered by a DNA replication fork, which leads to a structure that cannot be repaired by a DNA repair pathway (usually). a. What two cellular processes (or pathways or mechanisms) does the cell have to deal with this situation? b. What is the general name given to these two processes? 11.a. Name one protein that repairs 6-methylguanine (and 4-methylthymine). b. Begianing with ómeG, daw the mechanism. Do not draw guanine; simply begin with ICHy-G-DNAL a. When a glycosylase acts oa DNA damage il leaves b. The protein that acts on the entity mentioned in 12a. is aamed? c. The proteins in 12a. and 12b are part of the DNA repair pathway named 12. in DNA.

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indicale what the EcokI sate 2. For cach of the following blosung tew.hnius, complcte answers are possible, jusl give onac possabulity this taldc in your Bluc Book in any case muliple a Southcrn blot Masromalcul ingsi Tys.oruls 3. A 5000bp plasmid with no nicks is compleily B DNA and has 25 wnkerwindings What are L,T.W 4. What are the threce most impxtant negalive answers) 5. Name the kind of proicin thal: and AL taat uses for "egaavc sapcihelsal turus··thc cell? (Do NOT use Z-DNA as one d the a. Separates DNA strands b. Synthesizes RNA primers 6. a. The DaaA protcia cxists in an active and inactive form, which dilfer by binding to what small molecule(sy? b. The active form is? c. DaaA bound at oriC is converted to the inactive form by a process called what? d. DaaA NOT bound at oriC is converted to the inactive form at a site called what? 7. The timing of initiation of leading strand syathesis in E.coli can vary depcading on growh conditions in part from GTP (cither of its two aames is sufficient) (them) replication initiation. 8. Draw a figure (low chart) showing the steps during nonnal replication termination in E.coli, beginning after the a. Two sanall เ¡olecules can have an eflect on inaliauon of replicatia Name the small molecule that is derived b. This small nule cule is เ1ought lo allect DNA replication by anodulating thc cupression of a gcne(s), Name i c. la one sentence explain how the modulation of the gene (or one of the genes) you picked in 7.b. affects clockwise and counter-clockwise replication forks encounter each other. 9. The E.coli chromosome is compacted by binding to a variety of proteins. a. Name the proteia (or one of the proteins in the complex) that binds DNA to give -340bp loops b. This protcia (or prolein complex) is a member of what family of proteins? c. What cukaryotic ortholog is also in this family? 10. This concerns the fate of a lesion formed in DNA (e.g. a thymine dimer). If the DNA damage (lesion) is not repaired rapidly, then it might be encountered by a DNA replication fork, which leads to a structure that cannot be repaired by a DNA repair pathway (usually). a. What two cellular processes (or pathways or mechanisms) does the cell have to deal with this situation? b. What is the general name given to these two processes? 11.a. Name one protein that repairs 6-methylguanine (and 4-methylthymine). b. Begianing with ómeG, daw the mechanism. Do not draw guanine; simply begin with ICHy-G-DNAL a. When a glycosylase acts oa DNA damage il leaves b. The protein that acts on the entity mentioned in 12a. is aamed? c. The proteins in 12a. and 12b are part of the DNA repair pathway named 12. in DNA.

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