Question: Indicale whal the Ecoki site looks like when cach is acive 2. Fur cach of the tostlowing bMosung …


Please help it’s a test
indicale whal the Ecoki site looks like when cach is acive 2. Fur cach of the tostlowing bMosung to niqaes, complcte this tatdc in your B and Huc Book If in any case mulüple a. Southen blot b. Northe b c Wesicra blot A 5000bp plasmid with no nicks is completely B DNA and has 25 underwindings. What are L,T. W and AL 4. Whai are the three most important uses for uegative superhclical turns in the cel? (Do NOT use Z-DNA as one of the answes 5. Name the kind of proicin that: a. Separates DNA strands b Syathesizes RNA primern c Stabilizes ss-DNA (in bacieria). 6. a The DuaA proicia exists in an active and inactive form, which difler by binding to what small molecule(s? b. The active form is? c. DaaA bound at oriC is converted lto the inactive form by a process called what? d. DnaA NOT bound at oriC is coaverted to the inactive form at a site called what? 7. The timing of iaitiation of leading strand synthesis in E. coli can vary depending on growth conditions. a. Two small molecules can have an ellect on initiation of replication. Name the small molecule that is derived in part from GTP (cither of its iwo names is sufficient) b. This small molecule is thought to allect DNA replication by modulating the expression of a gene(s). Name it (them) c. la one sentence explain how the modulation of the gene (or one of the genes) you picked in 7.b. affects replication initiation 8. Draw a figure (low chart) showing the steps during nornal replication termination in E. coli, beginning after the clockwise and counter-clockwise replication forks encounter each other. 9. The E.coli chromosome is compacted by binding to a variety of protcins a. Name the protein (or one of the proteins in the complex) that binds DNA to give-340bp loops b. This protein (or protein complex) is a member of what family of proteins? c. What cukaryolic ortholog is also in this family? 10. This conceras the fate of a lesion formed in DNA (c.g. a thymine dimer). If the DNA damage (lesion) is not repaired rapidly, then it might be encountered by a DNA replication fork, which leads to a structure that cannot be repaired by a DNA repair pathway (usually). a. What two cellular processes (or pathways or mechanisms) does the cell have to deal with this situation? b. What is the general name given to these two processes? II.a. Name one protein that sepairs 6-mcthylguanine (and 4-methylthymine). b. Beginning with ómeG, draw the mechanism. Do not draw guanine; simply begin with [CH-G-DNAJ 12. a When a glycosylase acts on DNA damage it leaves in DNA. b. The protein that acts on the entity mentioned in 12a. is named c. The pruicias in 12a. and i 26, are part of the DNA repair pathway named

Show transcribed image text

indicale whal the Ecoki site looks like when cach is acive 2. Fur cach of the tostlowing bMosung to niqaes, complcte this tatdc in your B and Huc Book If in any case mulüple a. Southen blot b. Northe b c Wesicra blot A 5000bp plasmid with no nicks is completely B DNA and has 25 underwindings. What are L,T. W and AL 4. Whai are the three most important uses for uegative superhclical turns in the cel? (Do NOT use Z-DNA as one of the answes 5. Name the kind of proicin that: a. Separates DNA strands b Syathesizes RNA primern c Stabilizes ss-DNA (in bacieria). 6. a The DuaA proicia exists in an active and inactive form, which difler by binding to what small molecule(s? b. The active form is? c. DaaA bound at oriC is converted lto the inactive form by a process called what? d. DnaA NOT bound at oriC is coaverted to the inactive form at a site called what? 7. The timing of iaitiation of leading strand synthesis in E. coli can vary depending on growth conditions. a. Two small molecules can have an ellect on initiation of replication. Name the small molecule that is derived in part from GTP (cither of its iwo names is sufficient) b. This small molecule is thought to allect DNA replication by modulating the expression of a gene(s). Name it (them) c. la one sentence explain how the modulation of the gene (or one of the genes) you picked in 7.b. affects replication initiation 8. Draw a figure (low chart) showing the steps during nornal replication termination in E. coli, beginning after the clockwise and counter-clockwise replication forks encounter each other. 9. The E.coli chromosome is compacted by binding to a variety of protcins a. Name the protein (or one of the proteins in the complex) that binds DNA to give-340bp loops b. This protein (or protein complex) is a member of what family of proteins? c. What cukaryolic ortholog is also in this family? 10. This conceras the fate of a lesion formed in DNA (c.g. a thymine dimer). If the DNA damage (lesion) is not repaired rapidly, then it might be encountered by a DNA replication fork, which leads to a structure that cannot be repaired by a DNA repair pathway (usually). a. What two cellular processes (or pathways or mechanisms) does the cell have to deal with this situation? b. What is the general name given to these two processes? II.a. Name one protein that sepairs 6-mcthylguanine (and 4-methylthymine). b. Beginning with ómeG, draw the mechanism. Do not draw guanine; simply begin with [CH-G-DNAJ 12. a When a glycosylase acts on DNA damage it leaves in DNA. b. The protein that acts on the entity mentioned in 12a. is named c. The pruicias in 12a. and i 26, are part of the DNA repair pathway named

(Visited 1 times, 1 visits today)
Translate »