Question: /group/414c9613-6775-4a65-88d2-6d712d19ddct EXPERIMENT #2 Measuring Protein in Solution: The Brad…


/group/414c9613-6775-4a65-88d2-6d712d19ddct EXPERIMENT #2 Measuring Protein in Solution: The Bradford Assay 4 In this experiment spedifically, to determine the concentration of a protein sample (of a use Beers Law to determine the protein concentration based on its absorbance at 595 nm and molar absorption coefficient b. create a standard curve using a series of protein dilutions of known concentration, visually estimate the concentration of the unknown by where it als on the slope of the line /0.5 create a standard curve using a series of protein dilutions of known concentration and caloulate the concentration of the unknown by using the resulting slopes equation. d. use any of the above methods S. One grotein samgle of known concentration (0.5 imgm.)s used in this experiment Why? Brielly explain /0.5 6. What happened when you tested a sample of arginine with the Bradford assay? Did the Vdye react with this amino acid? Briely expiain why or why not /0.5 7. You also tested a food sample Compare the amount protein you measured for the Obety eo.n sample to the phished proton val. Ate vahes dose σ why or why not /0.5 Say you ran aprotein sample of unknown concentration four imes uing the Bradford assay and you find its concentralion s 5.2 7.1,29 and 03 mim for Mtrials 182 3 and 4, respectively. Likely you wouid conclude these e lack preoision and accuracy P lack precision, but are acourane If you plot the absorbance of each of the would be placed on the plot as follows 0.5 BIOLOGY 0

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/group/414c9613-6775-4a65-88d2-6d712d19ddct EXPERIMENT #2 Measuring Protein in Solution: The Bradford Assay 4 In this experiment spedifically, to determine the concentration of a protein sample (of a use Beer's Law to determine the protein concentration based on its absorbance at 595 nm and molar absorption coefficient b. create a standard curve using a series of protein dilutions of known concentration, visually estimate the concentration of the unknown by where it als on the slope of the line /0.5 create a standard curve using a series of protein dilutions of known concentration and caloulate the concentration of the unknown by using the resulting slope's equation. d. use any of the above methods S. One grotein samgle of known concentration (0.5 imgm.)s used in this experiment Why? Brielly explain /0.5 6. What happened when you tested a sample of arginine with the Bradford assay? Did the Vdye react with this amino acid? Briely expiain why or why not /0.5 7. You also tested a food sample Compare the amount protein you measured for the Obety eo.n sample to the phished proton val. Ate vahes dose σ why or why not /0.5 Say you ran aprotein sample of unknown concentration four imes uing the Bradford assay and you find its concentralion s 5.2 7.1,29 and 03 mim for Mtrials 182 3 and 4, respectively. Likely you wouid conclude these e lack preoision and accuracy P lack precision, but are acourane If you plot the absorbance of each of the would be placed on the plot as follows 0.5 BIOLOGY 0

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