Question: Find a picture of the ladder/marker we are using for the
experiment. Draw a figure of a gel with …


Find a picture of the ladder/marker we are using for the
experiment. Draw a figure of a gel with 10 lanes, with the
ladder/marker (the one we are using) in the first lane. Label the
lanes on the marker lane. In lane 2, draw what you expect a mixture
of proteins to look like (let’s say there are 1000 different
proteins with molecular weights between 2 kD and 130 kD). Think
about a smear rather than individual bands. In lane 3 draw what a
monomeric 18 kD protein would look like. In lane 4, draw what it
would look like if the protein in well 3 dimerized. In lane 5, draw
what it would look like if there were both monomers and dimers of
the 18 kD protein present. Remember DTT and 2-mercaptoethanol
reduce disulfide bonds and make monomers out of dimers etc.

(Visited 1 times, 1 visits today)
Translate »