Question: Embryonic liver tissue contains an enzyme that catalyzes the reaction S -> P. Adult liver display…


Embryonic liver tissue contains an enzyme that catalyzes the reaction S -> P. Adult liver displays the same activity. Some kinetic data is given below. Plot both a standard v vs. [S curve as well as a double reciprocal plot. Using the later plot calculate Vmax and Km. What do you notice about the relationships between Vmax and Km for the two enzymes? How will this effect the enzymatic activity in the adult vs. the embryonic liver? Please upload your excel file. Include in the file the answers to the above questions [Sl Initial Velocity umoles/mg/min Adult E1 1.05 1.54 1.98 2.86 3.78 5.00 6.67 7.15 8.00 10.00 Embryonic 1.67E-05 2.50E-05 3.33E-05 5.00E-05 7.00E-05 1.00E-04 1.50E-04 1.67E-04 2.00E-04 3.00E-04 E2 5.00 6.66 8.00 10.00 11.67 13.33 15.00 15.40 16.00 17.10

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Embryonic liver tissue contains an enzyme that catalyzes the reaction S -> P. Adult liver displays the same activity. Some kinetic data is given below. Plot both a standard v vs. [S curve as well as a double reciprocal plot. Using the later plot calculate Vmax and Km. What do you notice about the relationships between Vmax and Km for the two enzymes? How will this effect the enzymatic activity in the adult vs. the embryonic liver? Please upload your excel file. Include in the file the answers to the above questions [Sl Initial Velocity umoles/mg/min Adult E1 1.05 1.54 1.98 2.86 3.78 5.00 6.67 7.15 8.00 10.00 Embryonic 1.67E-05 2.50E-05 3.33E-05 5.00E-05 7.00E-05 1.00E-04 1.50E-04 1.67E-04 2.00E-04 3.00E-04 E2 5.00 6.66 8.00 10.00 11.67 13.33 15.00 15.40 16.00 17.10

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