Question: Answer to the best of your ability. To help think of the natural biological process may help you …


Answer to the best of your ability. To help think of the natural biological process may help you understand this technique. The primer used in Sanger sequencing A) can have any nucleotide sequence B) must have a sequence beginning and ending with the same nucleotide C) has a nucleotide sequence complementary to the 3 end of the region to be 1. copied (DNA) D) has a nucleotide sequence complementary to the 5 end of the region to be copied (DNA) 2. To carry out Sanger sequencing a mixture is needed containing A) B) c) D) single-stranded DNA DNA polymerase four deoxyribonucleotides A, T, C, G all of the above When a dideoxyribonucleotide is added to the tube A) replication of the strand continues B) replication of the strand stops C) replication of the strand is not affected D) replication of the strand is speeded up 3. Once the samples are loaded in machine the sequence of the DNA sample is read bottom to top one base at a time. Below is the image of a gel polymer. List the sequence of bases. 4. a. GTACCCGAAATCAGGA b. AGGACTAAAGCCCATG c. GTACCCGAATTCAGGA d. AGCACTAAAGCCCATG G A T C

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Answer to the best of your ability. To help think of the natural biological process may help you understand this technique. The primer used in Sanger sequencing A) can have any nucleotide sequence B) must have a sequence beginning and ending with the same nucleotide C) has a nucleotide sequence complementary to the 3' end of the region to be 1. copied (DNA) D) has a nucleotide sequence complementary to the 5' end of the region to be copied (DNA) 2. To carry out Sanger sequencing a mixture is needed containing A) B) c) D) single-stranded DNA DNA polymerase four deoxyribonucleotides A, T, C, G all of the above When a dideoxyribonucleotide is added to the tube A) replication of the strand continues B) replication of the strand stops C) replication of the strand is not affected D) replication of the strand is speeded up 3. Once the samples are loaded in machine the sequence of the DNA sample is read bottom to top one base at a time. Below is the image of a gel polymer. List the sequence of bases. 4. a. GTACCCGAAATCAGGA b. AGGACTAAAGCCCATG c. GTACCCGAATTCAGGA d. AGCACTAAAGCCCATG G A T C

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