Question: A PCR product is 3000 bp long. It contains an EcoR1 palindromic
restriction site at position 900 …


A PCR product is 3000 bp long. It contains an EcoR1 palindromic
restriction site at position 900 bp. After digestion, you obtain
two DNA bands. One is 900 bp long and the other of 2100 bp
long.

a) When ligated with T4 DNA ligase, list all DNA product sizes
produced.

b) Repeat but assume the EcoR1 site is replaced with a
non-palindromic restriction site.

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