Question: A. Calculate the molarity of a linearized 5-kb vector DNA if you
put 50 ng of the vector in a 20 …


a. Calculate the molarity of a linearized 5-kb vector DNA if you
put 50 ng of the vector in a 20 μL of ligation reaction. 1 bp of
dsDNA is 650 dalton. Show your calculation. 


b. Determine the mass of a 1-kb insert needed to achieve a 6:2
molar ratio of insert:vector using the above 5-kb vector in a 20 μL
ligation reaction. Show your calculation.

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