Question: 8. How does size exclusion column chromatography work in purifying proteins? f you had two protei…


8. How does size exclusion column chromatography work in purifying proteins? f you had two proteins, one small and another large protein, which protein will come out first from the column? What is affinity column chromatography? Give an example of how affinity can be used for protein purification? Why is it possible to separate proteins by mass using SDS-PAGE gel . 0. electrophoresis? 1. What does activity refer to in protein purification? How does activity differ from specific activity?

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8. How does size exclusion column chromatography work in purifying proteins? f you had two proteins, one small and another large protein, which protein will come out first from the column? What is affinity column chromatography? Give an example of how affinity can be used for protein purification? Why is it possible to separate proteins by mass using SDS-PAGE gel . 0. electrophoresis? 1. What does activity refer to in protein purification? How does activity differ from specific activity?

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