Question: 7. Below is a short hypothetical gene from a bacteria with promoter and terminator highlighted. […


7. Below is a short hypothetical gene from a bacteria with promoter and terminator highlighted. [Total 8 points] Promoter Terminator 50 10 20 30 40 5 -CCGTAT GTATGGCGGGGCCCTTATACTGCATGACACGATAGCCTGTCGGGGGG-3 3-GGCATATTTGCATACCGCCCCGGGAATATGACGTACTGTGCTATCGGACAGCCCCCC-5 For the following questions, assume that transcription begins immediately to the right of the last A-T base pair in the promoter sequence and ends immediately to the left of the first G-C base pair in the terminator 7a. Write the correct mRNA produced from the transcription of this DNA molecule (RNA polymerase travels from left to right). Indicate the corresponding 5 and 3 ends of the mRNA molecule. [4 points] 7b. Reference the codon table to write the sequence of the polypeptide assembled by the translation of the mRNA produced by the transcription of the DNA molecule shown above. Assume translation starts at the AUG codon closest to the 5 end of the mRNA and ends at the stop codon. Indicate the amino and the carboxyl ends. [4 points]

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7. Below is a short hypothetical gene from a bacteria with promoter and terminator highlighted. [Total 8 points] Promoter Terminator 50 10 20 30 40 5 '-CCGTAT GTATGGCGGGGCCCTTATACTGCATGACACGATAGCCTGTCGGGGGG-3 3'-GGCATATTTGCATACCGCCCCGGGAATATGACGTACTGTGCTATCGGACAGCCCCCC-5 For the following questions, assume that transcription begins immediately to the right of the last A-T base pair in the promoter sequence and ends immediately to the left of the first G-C base pair in the terminator 7a. Write the correct mRNA produced from the transcription of this DNA molecule (RNA polymerase travels from left to right). Indicate the corresponding 5' and 3' ends of the mRNA molecule. [4 points] 7b. Reference the codon table to write the sequence of the polypeptide assembled by the translation of the mRNA produced by the transcription of the DNA molecule shown above. Assume translation starts at the AUG codon closest to the 5' end of the mRNA and ends at the stop codon. Indicate the amino and the carboxyl ends. [4 points]

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