Question: 3. DNA sequencing The PCR amplification that occurs during a sequencing reaction results in linea…


3. DNA sequencing The PCR amplification that occurs during a sequencing reaction results in linear amplification, though conventional PCR amplification with two delineating primers gives exponential amplification. (/3 Marks) a. What does linear amplification mean? ( /1 Mark) b. How many strands of DNA are amplified from a one single double-stranded template after 25 cycles of (1) a conventional PCR amplification and (2) a sequencing PCR amplification?( /2 Marks)

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3. DNA sequencing The PCR amplification that occurs during a sequencing reaction results in linear amplification, though conventional PCR amplification with two delineating primers gives exponential amplification. (/3 Marks) a. What does linear amplification mean? ( /1 Mark) b. How many strands of DNA are amplified from a one single double-stranded template after 25 cycles of (1) a conventional PCR amplification and (2) a sequencing PCR amplification?( /2 Marks)

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