Question: 15- Which option BEST describes sticky ends by restriction enzymes B. Sticky ends A. Sticky ends …


15- Which option BEST describes sticky ends by restriction enzymes B. Sticky ends A. Sticky ends are DNA fragments that carry a higher charge than normal after they have been cleaved are DNA fragments cleaved by a restriction enzyme so that one strand is longer than the other C. Sticky ends are DNA fragments cleaved by a restriction enzyme so that both strands are the same length. D. Sticky ends are DNA fragments that attract a carbohydrate molecule to one end after being cleaved by a restriction enzyme 16- In an agarose gel DNA electrophoresis, the DNA fragments are separated based on their a. Density b. Size c. Electrical charge d. Nucleotides sequences e. All of the above 17. Primer PSil in GMO detection test 18. DNA std (DNA ladder) 19. Precipitate DNA 20. Magnesium a. A necessary cofactor for DNA polymerization b. Incubate in 50 °C c. Confirm plant-based sample d. Layer cold alcohol over cell extract e. To measure the size of PCR products Short answer and essay questions (55/50 points) он 2p 1-Name this molecule 2- Cutting DNA with a particular restriction enzyme producesthat can be separated by gel electrophoresis. 4p What is Super weeds 3 p 3- 4 GMO, bad or good? Discuss at least two of the reasoning of each proponent and opponent 8

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15- Which option BEST describes sticky ends by restriction enzymes B. Sticky ends A. Sticky ends are DNA fragments that carry a higher charge than normal after they have been cleaved are DNA fragments cleaved by a restriction enzyme so that one strand is longer than the other C. Sticky ends are DNA fragments cleaved by a restriction enzyme so that both strands are the same length. D. Sticky ends are DNA fragments that attract a carbohydrate molecule to one end after being cleaved by a restriction enzyme 16- In an agarose gel DNA electrophoresis, the DNA fragments are separated based on their a. Density b. Size c. Electrical charge d. Nucleotides sequences e. All of the above 17. Primer PSil in GMO detection test 18. DNA std (DNA ladder) 19. Precipitate DNA 20. Magnesium a. A necessary cofactor for DNA polymerization b. Incubate in 50 °C c. Confirm plant-based sample d. Layer cold alcohol over cell extract e. To measure the size of PCR products Short answer and essay questions (55/50 points) он 2p 1-Name this molecule 2- Cutting DNA with a particular restriction enzyme producesthat can be separated by gel electrophoresis. 4p What is Super weeds 3 p 3- 4 GMO, bad or good? Discuss at least two of the reasoning of each proponent and opponent 8

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