Question: 10. A reticulate lysate is a system that contains all the components required for translation (ri…


10. A reticulate lysate is a system that contains all the components required for translation (ribosomal subunits, tRNA, amino acids, etc and allows the reaction to be carried out in a test tube. The polypeptide that is synthesized from the system depends on the sequence of the RNA. For example adding the RNA 5AUGUUUUUUUUUUUU to the lysate directs the synthesis of Met-Phe-Phe-Phe Phe. 10a. If you add an antibiotic that blocks translocation of tRNA from the A site to the P site of the ribosome to the system, what will be the sequence of polypeptide produced by the same RNA? Explain your answer briefly. [4 points] 10b. You add a new antibiotic with unknown function to the lysate and no polypeptide was produced. Suggest a step in translation that is inhibited by the antibiotics. [4 points]

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10. A reticulate lysate is a system that contains all the components required for translation (ribosomal subunits, tRNA, amino acids, etc and allows the reaction to be carried out in a test tube. The polypeptide that is synthesized from the system depends on the sequence of the RNA. For example adding the RNA 5'AUGUUUUUUUUUUUU to the lysate directs the synthesis of Met-Phe-Phe-Phe Phe. 10a. If you add an antibiotic that blocks translocation of tRNA from the A site to the P site of the ribosome to the system, what will be the sequence of polypeptide produced by the same RNA? Explain your answer briefly. [4 points] 10b. You add a new antibiotic with unknown function to the lysate and no polypeptide was produced. Suggest a step in translation that is inhibited by the antibiotics. [4 points]

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