Question: 10/6/2018 Practice Problems Practice Problems Continued 1. Draw the structure of an RNA dinucleot…


10/6/2018 Practice Problems Practice Problems Continued 1. Draw the structure of an RNA dinucleotide with the sequence 5 5. Explain why Tm is a measure of stability of the 6. Predict how a change of pH from 7 to 9 would 7. Explain how a change in pH could help stabilize 8. Outline the evidence that stacking in 2. Draw the structure of pairing sugars and phosphates. Using standard conventions, indicate the phate (dAMP) base double helix. (UMP). Include nitrogenous bases of the be expected to change the Tm. Explain your bonds with dotted lines. 3. Suppose that you were analyzing the DNA of a deep-ocean bacterium You isolated the DNA from the bacterium and from yeast, in the same buffer and compared their tms. The higher tm than the yeast DNA. Consider the three that and answer the A form of DNA. interactions can result in different DNA I DNA had a l interactions nteractions. Which of these three may account for the difference in tm? Explain your answer. Draw the chemical structure of a single strand of DNA with the sequence AC, molecules having different Tms 4. Draw arrows to indicate which of the atoms of the nitrogenous bases

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10/6/2018 Practice Problems Practice Problems Continued 1. Draw the structure of an RNA dinucleotide with the sequence 5 5. Explain why Tm is a measure of stability of the 6. Predict how a change of pH from 7 to 9 would 7. Explain how a change in pH could help stabilize 8. Outline the evidence that stacking in 2. Draw the structure of pairing sugars and phosphates. Using standard conventions, indicate the phate (dAMP) base double helix. (UMP). Include nitrogenous bases of the be expected to change the Tm. Explain your bonds with dotted lines. 3. Suppose that you were analyzing the DNA of a deep-ocean bacterium You isolated the DNA from the bacterium and from yeast, in the same buffer and compared their tm's. The higher tm than the yeast DNA. Consider the three that and answer the A form of DNA. interactions can result in different DNA I DNA had a l interactions nteractions. Which of these three may account for the difference in tm? Explain your answer. Draw the chemical structure of a single strand of DNA with the sequence AC, molecules having different Tm's 4. Draw arrows to indicate which of the atoms of the nitrogenous bases

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