Question: 1.      Which of
the following is not a macronutrient required by a…

1.      Which of
the following is not a macronutrient required by all

(A)  carbon                                                              (B)  molybdenum

(C)phosphorus                                                       (D)hydrogen

(E)  nitrogen

2.      Typically,
which of the following nutrients would be needed at the lowest
concentration in a bacterial culture medium?

(A)  copper                                                              (B)  magnesium

(C)calcium                                                             (D)phosphorus

(E)  iron

3.      An
organism that uses organic compounds from its environment as carbon
sources while obtaining energy from light can best be described as

(A)  organotroph                                                      (B)  chemoautotroph

(C)photoheterotroph                                               (D)photoautotroph

(E)  lithotroph

4.      Which of
the following statements is true?

(A)  Most bacterial species can be cultured in the

(B)  Macronutrients are present in sufficient
quantities for growth as contaminants in water and glassware.

(C)All bacterial species require the same essential

(D)Bacterial species use a limited number of nutrient

(E)  All organisms require sulfur as a

5.      Some
bacteria live in deep sea vents where no light penetrates, yet are
still capable of producing their own reduced carbon (e.g. fixing
carbon dioxide). These organisms are classified as

(A)  photoheterotroph                                               (B)  photoautotroph

(C)chemoheterotroph                                             (D)chemoautotroph

(E)  organotroph

6.      Nitrogen
fixation is the conversion of

(A)  ammonium ion to nitrogen
gas.                          (B)  nitrogen
gas to ammonium ion.

(C)nitrate ion to nitrogen
gas.                                 (D)ammonium
ion to nitrate ion.

(E)  nitrate ion to ammonium ion.

7.      Which of
the following is true of complex media?

(A)  Bacteria grow faster on them than on synthetic

(B)  All the chemical components and their
concentrations are known.

(C)Complex media are the most difficult of all to prepare.

(D)Complex media can help determine the metabolic capacity of an

(E)  They are also called minimal media.

8.      Which of
the following types of growth media contains blood?

(A)  minimal
media                                                   (B)  complex

media                                                   (D)enriched

(E)  selective media

9.      The bile
salts and crystal violet in MacConkey lactose plates prevent the
growth of Gram-positives. These additives make the MacConkey plate

(A)  complex
medium.                                              (B)  defined

medium.                                               (D)selective

(E)  differential medium.

10.    You discover a new
bacterial efflux pump that moves an uncharged toxin out of the cell
using the proton motive force as the energy source. This new
transporter can be classified as an

(A)  electrogenic
antiporter                                       (B)  electrogenic

antiporter                                     (D)electroneutral

(E)  none of the above

11.    Which of the
following involves nitrate as a terminal electron acceptor?

(A)  photosynthesis                                                  (B)  aerobic

(C)fermentation                                                     (D)anaerobic

(E)  glycolysis

12.    Which of the
following is least likely to use glucose (an organic molecule) as a
source of electrons?

(A)  lithotrophy                                                         (B)  organotrophy

respiration                                             (D)anaerobic

(E)  heterotrophy

13.    Identify the energy
source associated with the PTS system, a type of group
translocation, in which the substance transported across the cell
membrane is chemically modified.

(A)  ATP                                                                  (B)  Proton
motive force

(C)NADH                                                               (D)Phosphoenolpyruvate

(E)  Glucose-6-phosphate

14.    Which of the
following statements is FALSE about the phosphotransferase system

(A)  The Enzyme I complex is shared among all PTS

(B)  The Enzyme II complex is specific to the sugar
being transported.

(C)Technically, PTS does not transport a sugar against its
concentration gradient.

(D)The Enzyme I complex is in the cytoplasm, while the Enzyme II
complex is associated with the cell membrane.

(E)  All of the above are true statements.

15.    Siderophores are
responsible for helping bacteria acquire

(A)  ammonium
ion.                                                 (B)  phosphorus.

(C)iron.                                                                  (D)calcium.

(E)  glucose.

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