Question: 1. Diagram and briefly explain the reduction reaction between B-mercaptoethanol and the disulfide…


1. Diagram and briefly explain the reduction reaction between B-mercaptoethanol and the disulfide bond linking the 2 subunits of protein X whose subunits are 75kD and 25kD 2. Diagram the SDS-PAGE gel of the above protein X when run under 2 different conditions. (a) with the addition of BME, (b) without the addition of BME. Be sure to include a ladder with the MW in kD. This does not need to be exact or perfect. A quick sketch is all that is required
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1. Diagram and briefly explain the reduction reaction between B-mercaptoethanol and the disulfide bond linking the 2 subunits of protein X whose subunits are 75kD and 25kD 2. Diagram the SDS-PAGE gel of the above protein X when run under 2 different conditions. (a) with the addition of BME, (b) without the addition of BME. Be sure to include a ladder with the MW in kD. This does not need to be exact or perfect. A quick sketch is all that is required

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