Question: 1. (30 pts). The figure below is error free data for the binding of a ligand L to a protein P. Th…


1. (30 pts). The figure below is error free data for the binding of a ligand L to a protein P. The protein concentration used in generating these data is 1 μΜ. Estimate the value for KL and the number of ligands bound per protein molecule at saturation. Use your estimate for the value of K to calculate the average number of bound ligands at each value of Lo 0, 0.125, 0.25, 0.5 1.0, 2.0, and 4.0 HM. Does your calculated data come close to the observed data? If not, something must be wrong. Figure out what is wrong and make it right. Observed data 1.20 1.00 0.80 0.60 0.40 0.20 0.00 0.0000 0.5000 1.0000 1.5000 2.0000 2.5000 3.0000 3.5000 4.0000 4.5000 Lo μΜ Lo HM 0.0000 0.1250 0.2500 0.5000 1.0000 2.0000 4.0000 PL]/Po observed 0.00 0.12 0.25 0.49 0.90 0.99 1.00

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1. (30 pts). The figure below is error free data for the binding of a ligand L to a protein P. The protein concentration used in generating these data is 1 μΜ. Estimate the value for KL and the number of ligands bound per protein molecule at saturation. Use your estimate for the value of K to calculate the average number of bound ligands at each value of Lo 0, 0.125, 0.25, 0.5 1.0, 2.0, and 4.0 HM. Does your calculated data come close to the observed data? If not, something must be wrong. Figure out what is wrong and make it right. Observed data 1.20 1.00 0.80 0.60 0.40 0.20 0.00 0.0000 0.5000 1.0000 1.5000 2.0000 2.5000 3.0000 3.5000 4.0000 4.5000 Lo μΜ Lo HM 0.0000 0.1250 0.2500 0.5000 1.0000 2.0000 4.0000 PL]/Po observed 0.00 0.12 0.25 0.49 0.90 0.99 1.00

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