Question: 0o0N any of 8- What would be the effect on the PCR 1) there are no primers in the reaction 2) the…


0o0N any of 8- What would be the effect on the PCR 1) there are no primers in the reaction 2) there are no dNTPs in the reaction, 3) there is no Taq połymerase in the reaction? reaction if any of the following circumstances arose: a. PCR would proceed normally b. Non-specific PCR of random templates will occur c. The reaction will cease after a few cycles d. The PCR reaction will not commence 9- Which of the following is NOT true about Taq DNA polymerase? A. It functions at extremely high temperatures B. It adds nucleotide triphosphates to form a new DNA strand C. It requires a salt environment (buffer) to work D. It can act as an exonuclease to cut single-stranded DNA 10- Why you add Nucleotide triphosphates (dNTPs) in PCR reaction mix while you need dNMPs to add to growing DNA strand A. To increase the rate of reaction by using bigser nucleotide B. better annealing process C stronger newly made DNA D. use the energy from third phosphate bond for polymerization 11- In a RT-PCR reaction Nucleotide triphosphates (dNTPs) are added to the growingby A. RNA, polymerase enzyme, B. RNA, restriction enzyme C. DNA, reverse transcriptase D. RNA, reverse transcriptase 12- What type of PCR determine the amount of initial DNA in sample. A. Real Time B. Allele Specific C. Multiplex D. RT-PCR 13- All restriction enzyme recognition sites A. Are never read the same forward and backward. B. End with CGT C. Begin with CGT D. Are read the same forward and backward. 14- BamHl, Hindill, and EcoRI are all examples of A. Genes. B.DNA ligands. C. Restriction enzymes.D. Southern blots.

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0o0N any of 8- What would be the effect on the PCR 1) there are no primers in the reaction 2) there are no dNTPs in the reaction, 3) there is no Taq połymerase in the reaction? reaction if any of the following circumstances arose: a. PCR would proceed normally b. Non-specific PCR of random templates will occur c. The reaction will cease after a few cycles d. The PCR reaction will not commence 9- Which of the following is NOT true about Taq DNA polymerase? A. It functions at extremely high temperatures B. It adds nucleotide triphosphates to form a new DNA strand C. It requires a salt environment (buffer) to work D. It can act as an exonuclease to cut single-stranded DNA 10- Why you add Nucleotide triphosphates (dNTPs) in PCR reaction mix while you need dNMPs to add to growing DNA strand A. To increase the rate of reaction by using bigser nucleotide B. better annealing process C stronger newly made DNA D. use the energy from third phosphate bond for polymerization 11- In a RT-PCR reaction Nucleotide triphosphates (dNTPs) are added to the growingby A. RNA, polymerase enzyme, B. RNA, restriction enzyme C. DNA, reverse transcriptase D. RNA, reverse transcriptase 12- What type of PCR determine the amount of initial DNA in sample. A. Real Time B. Allele Specific C. Multiplex D. RT-PCR 13- All restriction enzyme recognition sites A. Are never read the same forward and backward. B. End with CGT C. Begin with CGT D. Are read the same forward and backward. 14- BamHl, Hindill, and EcoRI are all examples of A. Genes. B.DNA ligands. C. Restriction enzymes.D. Southern blots.

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